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Antimicrobial Agents and Chemotherapy, April 2002, p. 1132-1135, Vol. 46, No. 4
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.4.1132-1135.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning and Expression of Class A ß-Lactamase Gene blaA_BPS in Burkholderia pseudomallei

Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China

Received 7 June 2001/ Returned for modification 29 September 2001/ Accepted 13 January 2002

The ß-lactamase gene blaA_BPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A ß-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.

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