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Antimicrobial Agents and Chemotherapy, May 2003, p. 1584-1588, Vol. 47, No. 5
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.5.1584-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Aminoglycoside Resistance Gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a Clinical Isolate from Bulgaria

Stefana Sabtcheva,1,{dagger} Marc Galimand,1 Guy Gerbaud,1 Patrice Courvalin,1* and Thierry Lambert1,2

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,1 Centre d'Etudes Pharmaceutiques, 92296 ChÂtenay-Malabry, France2

Received 14 November 2002/ Returned for modification 7 January 2003/ Accepted 11 February 2003

The ant(4')-IIb gene of Pseudomonas aeruginosa BM4492, which encodes an aminoglycoside 4'-O-adenylyltransferase, was identified as a coding sequence of 756 bp corresponding to a protein with a calculated mass of 27,219 Da. Analysis of the deduced sequence indicated that the protein was related to aminoglycoside 4'-O-adenylyltransferases IIa and Ia found in P. aeruginosa and gram-positive bacteria, respectively. The enzyme conferred resistance to amikacin and tobramycin but not to dibekacin, gentamicin, or netilmicin. The ant(4')-IIb gene had a chromosomal location in five of six clinical isolates of P. aeruginosa tested and was plasmid borne in the remaining strain. The ant(4')-IIb gene was detected by PCR in some clinical strains of P. aeruginosa from the same hospital but not in members of other bacterial genera.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 45 68 83 20. Fax: (33) 1 45 68 83 19. E-mail: pcourval{at}pasteur.fr.

{dagger} Present address: Laboratory for Clinical Microbiology, National Oncology Center, 1756 Sofia, Bulgaria.

Antimicrobial Agents and Chemotherapy, May 2003, p. 1584-1588, Vol. 47, No. 5
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.5.1584-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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