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Antimicrobial Agents and Chemotherapy, December 2008, p. 4478-4482, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.01072-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

MexCD-OprJ Multidrug Efflux System of Pseudomonas aeruginosa: Involvement in Chlorhexidine Resistance and Induction by Membrane-Damaging Agents Dependent upon the AlgU Stress Response Sigma Factor

Sebastien Fraud, Aaron J. Campigotto, Zhilin Chen, and Keith Poole^*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Received 8 August 2008/ Returned for modification 7 September 2008/ Accepted 27 September 2008

The biocide chlorhexidine (CHX) as well as additional membrane-active agents were shown to induce expression of the mexCD-oprJ multidrug efflux operon, dependent upon the AlgU stress response sigma factor. Hyperexpression of this efflux system in nfxB mutants was also substantially AlgU dependent. CHX resistance correlated with efflux gene expression in various mutants, consistent with MexCD-OprJ being a determinant of CHX resistance.

Published ahead of print on 6 October 2008.

Both authors contributed equally to this work.