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Antimicrobial Agents and Chemotherapy, November 2009, p. 4911-4914, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00703-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Detection of a Point Mutation in the parC Gene Associated with Decreased Susceptibility to Fluoroquinolones in Mycoplasma bovis{triangledown}

I. Lysnyansky,* I. Mikula, I. Gerchman, and S. Levisohn

Mycoplasma Unit, Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel

Received 26 May 2009/ Returned for modification 10 July 2009/ Accepted 22 August 2009

Comparison of the quinolone resistance-determining regions (QRDRs) in 42 Mycoplasma bovis clinical isolates revealed amino acid substitutions at both GyrA (position 83) and ParC (position 84) in 10/11 enrofloxacin-resistant strains. The mutation present in the parC QRDR was discriminative for enrofloxacin resistance by parC PCR-restriction fragment length polymorphism. Comparison of molecular profiles by insertion sequence typing suggests that the currently prevalent enrofloxacin-resistant M. bovis strain evolved by selection under field conditions from one of the susceptible strains.

* Corresponding author. Mailing address: Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, POB 12, Bet Dagan 50250, Israel. Phone: 972 3 9681617. Fax: 972 3 9681739. E-mail: innal{at}moag.gov.il

{triangledown} Published ahead of print on 31 August 2009.

Antimicrobial Agents and Chemotherapy, November 2009, p. 4911-4914, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00703-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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