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Antimicrobial Agents and Chemotherapy, November 2009, p. 4930-4933, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00900-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Nosocomial Spread of Colistin-Only-Sensitive Sequence Type 235 Pseudomonas aeruginosa Isolates Producing the Extended-Spectrum β-Lactamases GES-1 and GES-5 in Spain{triangledown}

Esther Viedma,1 Carlos Juan,2 Joshi Acosta,1 Laura Zamorano,2 Joaquín R. Otero,1 Francisca Sanz,1 Fernando Chaves,1 and Antonio Oliver2*

Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain,1 Servicio de Microbiología and Unidad de Investigación, Instituto Universitario de Investigación en Ciencias de la Salud (IUNICS), Palma de Mallorca, Spain2

Received 1 July 2009/ Returned for modification 14 August 2009/ Accepted 29 August 2009

The mechanisms responsible for the increasing prevalence of colistin-only-sensitive (COS) Pseudomonas aeruginosa isolates in a Spanish hospital were investigated. Pulsed-field gel electrophoresis revealed that 24 (50%) of the studied isolates belonged to the same clone, identified as the internationally spread sequence type 235 (ST235) through multilocus sequence typing. In addition to several mutational resistance mechanisms, an integron containing seven resistance determinants was detected. Remarkably, the extended-spectrum β-lactamase GES-1 and its Gly170Ser carbapenem-hydrolyzing derivative GES-5 were first documented to be encoded in a single integron. This work is the first to describe GES enzymes in Spain and adds them to the growing list of β-lactamases of concern (PER, VIM, and OXA) detected in ST235 clone isolates.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Son Dureta, C. Andrea Doria N° 55, 07014 Palma de Mallorca, Spain. Phone: 34 971 175 148. Fax: 34 971 175 185. E-mail: antonio.oliver{at}ssib.es

{triangledown} Published ahead of print on 8 September 2009.

Antimicrobial Agents and Chemotherapy, November 2009, p. 4930-4933, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00900-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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