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Antimicrobial Agents and Chemotherapy, November 2009, p. 4944-4948, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00787-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Combined Inactivation of lon and ycgE Decreases Multidrug Susceptibility by Reducing the Amount of OmpF Porin in Escherichia coli{triangledown}

Valérie Duval, Hervé Nicoloff,{dagger} and Stuart B. Levy*

Center for Adaptation Genetics and Drug Resistance and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111

Received 12 June 2009/ Returned for modification 3 July 2009/ Accepted 22 August 2009

Transposon inactivation of ycgE, a gene encoding a putative transcriptional regulator, led to decreased multidrug susceptibility in an Escherichia coli lon mutant. The multidrug susceptibility phenotype (e.g., to tetracycline and β-lactam antibiotics) required the inactivation of both lon and ycgE. In this mutant, a decreased amount of OmpF porin contributes to the lowered drug susceptibility, with a greater effect at 26°C than at 37°C.

* Corresponding author. Mailing address: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111. Phone: (617) 636-6764. Fax: (617) 636-0458. E-mail: stuart.levy{at}tufts.edu

{triangledown} Published ahead of print on 31 August 2009.

{dagger} Present address: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802.

Antimicrobial Agents and Chemotherapy, November 2009, p. 4944-4948, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00787-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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